GSM5172710: Xbp1fl/fl; Mus musculus; RNA-Seq - SRA

SRX10344551: GSM5172710: Xbp1fl/fl; Mus musculus; RNA-Seq
2 ILLUMINA (Illumina NovaSeq 6000) runs: 219.2M spots, 27.8G bases, 8.3Gb downloads

Submitted by: NCBI (GEO)

Study: Droplet-based single-cell RNA-sequencing facilitates trancritption analysis of small intestine CD4 cells from Germline free Xbp1?IEC and Xbp1fl/fl

PRJNA714643 • SRP310713 • All experiments • All runs

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We performed theDroplet-based single-cell RNA-sequencing on small intestine CD4 cells from Germline free(GF) Xbp1?IEC and Xbp1fl/fl mice and found the effect of ER stress in epithelia cells on CD4 cells. Overall design: SI LP CD4 (CD45+CD4+TCRß+) living cells from Germline free Xbp1?IEC and Xbp1fl/fl were FACS-sorted for sequencing. The sorted live cells were separated into a single droplet using the Chromium Single Cell 3' v3 kit according to the manufacturer's instruction (10x Genomics). The libraries were sequenced on NovaSeq 6000 S2 platform.

Sample: Xbp1fl/fl

SAMN18314033 • SRS8463668 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: 10k SI LP CD4 (CD45+CD4+TCRβ+) living cells from Germline free Xbp1ΔIEC and Xbp1fl/fl were FACS-sorted for sequencing. The sorted live cells were separated into a single droplet using the Chromium Single Cell 3' v3 kit according to the manufacturer's instruction (10x Genomics) and RNA libraries were prepared for sequencing using standard protocols.

Experiment attributes:

GEO Accession: GSM5172710

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 219.2M spots, 27.8G bases, 8.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR13966624	110,048,572	14G	4.2Gb	2023-03-16
SRR13966625	109,172,307	13.9G	4.1Gb	2023-03-16

ID:: 13537503

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